Can rouge use Assassinate twice if there is a surprise round and has higher initiative than the target?

@JeremyECrawford Can a rouge use Assassinate twice if there is a surprise round and the rouge has higher initiative than the target?

— Spike229 (@spike229) October 15, 2016

Assassinate works only during the first round of a combat, unless a creature joins a fight later and has a lower initiative. #DnD https://t.co/A3bgPIfTCF

— Jeremy Crawford (@JeremyECrawford) October 17, 2016